Product Name

Beclin 1 antibody

Documents

Description

Beclin 1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in MDA-MB-453s cells, A431 cells, HEK-293 cells, HeLa cells, HT-1080 cells, human placenta tissue, L02 cells, mouse brain tissue, NIH/3T3 cells, PC-3 cells, Raji cells, rat brain tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 60 kDa

Tested applications

ELISA, IHC, IP, WB, IF

Species reactivity

Human, Mouse, Rat; other species not tested.

Alternative names

ATG6 antibody; Beclin 1 antibody; beclin 1 antibody; autophagy related antibody; beclin1 antibody; BECN1 antibody; GT197 antibody; Protein GT197 antibody; VPS30 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Beclin 1 recombinant protein (Accession Number: NM_001313998). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:117114(Beclin 1 Antibody) at dilution of 1:1000

IP Result of anti-Beclin 1 (IP:Catalog No:117114, 3ug; Detection:Catalog No:117114 1:500) with HeLa cells lysate 1200ug.

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117114(Beclin 1 antibody) at dilution of 1:50 (under 10x lens)

Immunofluorescent analysis of NIH/3T3 cells using Catalog No:117114(Beclin 1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

Beclin-1 (BECN1), also known as ATG6 or VPS30, has a central role in autophagy, a process of programmed cell survival, which is increased during periods of cell stress and extinguished during the cell cycle. Beclin-1, may play a role in antiviral host defense. It protects against infection by a neurovirulent strain of Sindbis virus. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. A progressively reduced Beclin-1 expression is reported to correlate with the primary tumor growth of squamous cell carcinoma and adenocarcinoma of the lung. Caspase-mediated cleavage of Beclin 1 promotes crosstalk between apoptosis and autophagy.

References

Fogel AI, Dlouhy BJ, Wang C. Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy. Molecular and cellular biology. 33(18):3675-88. 2013.
Feng W, Chang C, Luo D. Dissection of autophagy in human platelets. Autophagy. 10(4):642-51. 2014.
Guo L, Xu JM, Mo XY. Ischemic postconditioning regulates cardiomyocyte autophagic activity following ischemia/reperfusion injury. Molecular medicine reports. 12(1):1169-76. 2015.
Li J, Wang F, Xia Y. Astaxanthin Pretreatment Attenuates Hepatic Ischemia Reperfusion-Induced Apoptosis and Autophagy via the ROS/MAPK Pathway in Mice. Marine drugs. 13(6):3368-87. 2015.
Liu M, Xu L, Yin L. Potent effects of dioscin against obesity in mice. Scientific reports. 5:7973. 2015.
Song BQ, Chi Y, Li X. Inhibition of Notch Signaling Promotes the Adipogenic Differentiation of Mesenchymal Stem Cells Through Autophagy Activation and PTEN-PI3K/AKT/mTOR Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 36(5):1991-2002. 2015.
Li J, Yang D, Wang W. Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells. Journal of experimental & clinical cancer research : CR. 34:97. 2015.
Du TT, Wang L, Duan CL. GBA deficiency promotes SNCA/α-synuclein accumulation through autophagic inhibition by inactivated PPP2A. Autophagy. 11(10):1803-20. 2015.

Beclin 1 antibody

Product Information

Related Products / Services