Beclin 1 antibody

Cat.#: 108441

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Product Information

  • Product Name
    Beclin 1 antibody
  • Documents
  • Description
    Beclin 1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in MDA-MB-453s cells, A431 cells, HEK-293 cells, HeLa cells, HT-1080 cells, human placenta tissue, L02 cells, mouse brain tissue, NIH/3T3 cells, PC-3 cells, Raji cells, rat brain tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 60 kDa
  • Tested applications
    ELISA, IHC, IP, WB, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    ATG6 antibody; Beclin 1 antibody; beclin 1 antibody; autophagy related antibody; beclin1 antibody; BECN1 antibody; GT197 antibody; Protein GT197 antibody; VPS30 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Beclin 1 recombinant protein (Accession Number: NM_001313998). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:117114(Beclin 1 Antibody) at dilution of 1:1000

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:117114(Beclin 1 Antibody) at dilution of 1:1000

    IP Result of anti-Beclin 1 (IP:Catalog No:117114, 3ug; Detection:Catalog No:117114 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-Beclin 1 (IP:Catalog No:117114, 3ug; Detection:Catalog No:117114 1:500) with HeLa cells lysate 1200ug.

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117114(Beclin 1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117114(Beclin 1 antibody) at dilution of 1:50 (under 10x lens)

    Immunofluorescent analysis of NIH/3T3 cells using Catalog No:117114(Beclin 1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of NIH/3T3 cells using Catalog No:117114(Beclin 1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Beclin-1 (BECN1), also known as ATG6 or VPS30, has a central role in autophagy, a process of programmed cell survival, which is increased during periods of cell stress and extinguished during the cell cycle. Beclin-1, may play a role in antiviral host defense. It protects against infection by a neurovirulent strain of Sindbis virus. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. A progressively reduced Beclin-1 expression is reported to correlate with the primary tumor growth of squamous cell carcinoma and adenocarcinoma of the lung. Caspase-mediated cleavage of Beclin 1 promotes crosstalk between apoptosis and autophagy.
  • References
    • Fogel AI, Dlouhy BJ, Wang C. Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy. Molecular and cellular biology. 33(18):3675-88. 2013.
    • Feng W, Chang C, Luo D. Dissection of autophagy in human platelets. Autophagy. 10(4):642-51. 2014.
    • Guo L, Xu JM, Mo XY. Ischemic postconditioning regulates cardiomyocyte autophagic activity following ischemia/reperfusion injury. Molecular medicine reports. 12(1):1169-76. 2015.
    • Li J, Wang F, Xia Y. Astaxanthin Pretreatment Attenuates Hepatic Ischemia Reperfusion-Induced Apoptosis and Autophagy via the ROS/MAPK Pathway in Mice. Marine drugs. 13(6):3368-87. 2015.
    • Liu M, Xu L, Yin L. Potent effects of dioscin against obesity in mice. Scientific reports. 5:7973. 2015.
    • Song BQ, Chi Y, Li X. Inhibition of Notch Signaling Promotes the Adipogenic Differentiation of Mesenchymal Stem Cells Through Autophagy Activation and PTEN-PI3K/AKT/mTOR Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 36(5):1991-2002. 2015.
    • Li J, Yang D, Wang W. Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells. Journal of experimental & clinical cancer research : CR. 34:97. 2015.
    • Du TT, Wang L, Duan CL. GBA deficiency promotes SNCA/α-synuclein accumulation through autophagic inhibition by inactivated PPP2A. Autophagy. 11(10):1803-20. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"