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Product Name
Anti-Beclin 1 Rabbit antibody
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Description
Beclin 1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Beclin 1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/20
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Validations
Western blot analysis of extracts from 3T3, K562, MCF7 and Jurkat cells using Beclin 1 Rabbit pAb at 1:1000 dilution. Predicted band size: 55kDa. Observed band size: 60kDa.
Western blot analysis of Beclin 1 expression in (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
Immunofluorescent analysis of Hela cells, using Beclin 1 Antibody.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Beclin 1 Antibody.
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Background
Swiss-Prot Acc.Q14457.Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
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References
- Metformin prevents against oxidative stress-induced senescence in human periodontal ligament cells
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"