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Product Name
B23 antibody
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Description
B23 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in multi-cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells. Positive IHC detected in human colon cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 35-40kd
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
B23 antibody; NPM1 antibody; Nucleolar phosphoprotein B23 antibody; Nucleolar protein NO38 antibody; Nucleophosmin antibody; Numatrin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of B23 recombinant protein (Accession Number: NM_001355006). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
WB result of anti-NPM1 (Catalog No:117084) in different cell lysates.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117084(NPM1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117084(NPM1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-B23 (IP:Catalog No:117084, 4ug; Detection:Catalog No:117084 1:2000) with Jurkat cells lysate 3200ug.
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Background
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
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References
- Guo Y, Liu S, Wang P. Granulocyte colony-stimulating factor improves neuron survival in experimental spinal cord injury by regulating nucleophosmin-1 expression. Journal of neuroscience research. 92(6):751-60. 2014.
- Szerlong HJ, Herman JA, Krause CM. Proteomic characterization of the nucleolar linker histone H1 interaction network. Journal of molecular biology. 427(11):2056-71. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"