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Product Name
B23 antibody
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Description
B23 Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 33kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
B23 antibody; NPM antibody; NPM1 antibody; Nucleophosmin antibody; Numatrin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of B23 recombinant protein (Accession Number: NM_001355006). Purification method: Caprylic Acid/Ammonium Sulfate Precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107075(B23 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107075(B23 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107075(B23 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using B23 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
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References
- Jiang WQ, Nguyen A, Cao Y, Chang AC, Reddel RR. HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a. PloS one. 6(2):e17036. 2011.
- Szerlong HJ, Herman JA, Krause CM. Proteomic characterization of the nucleolar linker histone H1 interaction network. Journal of molecular biology. 427(11):2056-71. 2015.
- Tao Z, Wang H, Xia Q. Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity. Human molecular genetics. 24(9):2426-41. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"