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Anti-Nucleophosmin (7H10) Mouse antibody

Cat.#: 168298

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Product Information

  • Product Name
    Anti-Nucleophosmin (7H10) Mouse antibody
  • Documents
  • Description
    Nucleophosmin (7H10) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, IP
  • Species reactivity
    Human, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human NPM (2-265) expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    ELISA: 1/10000

  • Validations

    Immunoprecipitation analysis of Hela cell lysates using Nucleophosmin mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using Nucleophosmin mouse mAb.

    Western blot detection of Nucleophosmin in HaCat,COS7,293,HepG2 and Hela cell lysates using NPM1 mouse mAb (dilution 1:500).Predicted band size:33 Kda.Observed band size:38KDa.

    Western blot detection of Nucleophosmin in HaCat,COS7,293,HepG2 and Hela cell lysates using NPM1 mouse mAb (dilution 1:500).Predicted band size:33 Kda.Observed band size:38KDa.

    Immunocytochemistry of HeLa cells using Nucleophosmin mouse mAb (dilution 1:100).

    Immunocytochemistry of HeLa cells using Nucleophosmin mouse mAb (dilution 1:100).

  • Background
    Swiss-Prot Acc.P06748.Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"