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Product Name
APPL1 antibody
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Description
APPL1 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, mouse ovary tissue. Observed molecular weight by Western-blot: 80kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
APPL antibody; APPL1 antibody; Dip13 alpha antibody; DIP13A antibody; DIP13alpha antibody; KIAA1428 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of APPL1 recombinant protein (Accession Number: NM_012096). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108042(APPL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:108042(APPL1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-APPL1 (IP:Catalog No:108042, 4ug; Detection:Catalog No:108042 1:500) with mouse brain tissue lysate 4000ug.
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Background
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of insulin signaling, plays a key role in the regulation of insulin secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the insulin-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
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References
- Ogawa A, Yamazaki Y, Nakamori M. Characterization and distribution of adaptor protein containing a PH domain, PTB domain and leucine zipper motif (APPL1) in Alzheimer's disease hippocampus: an immunohistochemical study. Brain research. 1494:118-24. 2013.
- Flores-Rodriguez N, Kenwright DA, Chung PH. ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome. Journal of cell science. 128(4):755-67. 2015.
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