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Product Name
Anti-RhoGDI (3E9) Mouse antibody
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Description
RhoGDI (3E9) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue
Western blot detection of RhoGDI in C6,Jurkat,K562,3T3 and Hela cell lysates using RhoGDI mouse mAb (1:2000 diluted).Predicted band size:26KDa.Observed band size:26KDa.
Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).
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Background
Swiss-Prot Acc.P52565.Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"