Product Name

RhoGDI antibody

Documents

Description

RhoGDI Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A431 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse pancreas tissue. Positive IHC detected in human breast cancer tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 28 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

aGDI antibody; ARHGDIA antibody; GDIA1 antibody; Rho GDI 1 antibody; Rho GDI alpha antibody; RHOGDI antibody; RHOGDI 1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of RhoGDI recombinant protein (Accession Number: NM_004309). Purification method: Protein A purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114663(ARHGDIA,aGDI antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114663(ARHGDIA,aGDI antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114663(ARHGDIA,aGDI antibody) at dilution of 1:50 (under 40x lens)

Background

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (also called Rho GDP-dissociation inhibitor 1, Rho GDI1). Rho GDI1 acts as a metastasis-related protein in colon and prostate cancer patients and may be useful as a diagnostic biomarker to prevent colon and prostate cancer metastasis.

References

Song Q, Xu Y, Yang C. miR-483-5p promotes invasion and metastasis of lung adenocarcinoma by targeting RhoGDI1 and ALCAM. Cancer research. 74(11):3031-42. 2014.
Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
Zeng Z, Bo H, Gong Z. AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
Bo H, Gong Z, Zhang W. Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 6(24):20404-18. 2015.
Lichtenfels R, Mougiakakos D, Johansson CC. Comparative expression profiling of distinct T cell subsets undergoing oxidative stress. PloS one. 7(7):e41345. 2012.
Wang J, Wang J, Zhang Y. Proteomic analysis of adrenocorticotropic hormone treatment of an infantile spasm model induced by N-methyl-D-aspartic acid and prenatal stress. PloS one. 7(9):e45347. 2012.
Seyfried NT, Gozal YM, Donovan LE. Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards. Journal of proteome research. 11(5):2721-38. 2012.

RhoGDI antibody

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