Anti-Phospho-Hsp27 (Ser78) Rabbit antibody

Cat.#: 167122

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-Phospho-Hsp27 (Ser78) Rabbit antibody
  • Documents
  • Description
    Phospho-Hsp27 (Ser78) Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Peptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27.
  • Clonality
    Polyclonal
  • Formulation
    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100

  • Validations

    Western blot analysis of extracts from Hela cells, untreated or treated with UV, using HSP27 (Phospho-Ser78) Rabbit pAb (167122,1:500 diluted,upper) or Hsp27 Mouse mAb (200778,lower).

    Western blot analysis of extracts from Hela cells, untreated or treated with UV, using HSP27 (Phospho-Ser78) Rabbit pAb (167122,1:500 diluted,upper) or Hsp27 Mouse mAb (200778,lower).

    Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) Antibody .

    Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) Antibody .

    Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) Antibody .

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) Antibody (left) or the same antibody preincubated with blocking peptide (right).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) Antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Background
    Swiss-Prot Acc.P04792.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"