Anti-Hsp27 (9G1) Mouse antibody

Cat.#: 168173

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Product Information

  • Product Name
    Anti-Hsp27 (9G1) Mouse antibody
  • Documents
  • Description
    Hsp27 (9G1) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant protein expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1;000-2;000

    IHC: 1/100-200

  • Validations

    Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using HSP27 Mouse mAb diluted at 1:200.

    Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using HSP27 Mouse mAb diluted at 1:200.

    Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2,000.

    Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2,000.

    Western blot detection of HSP27 in K562,Hela cell lysates using HSP27 (9G1) Mouse mAb(1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa.

    Western blot detection of HSP27 in K562,Hela cell lysates using HSP27 (9G1) Mouse mAb(1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa.

  • Background
    Swiss-Prot Acc.P04792.Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"