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Product Name
Anti-Hsp27 (7E5) Mouse antibody
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Description
Hsp27 (7E5) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Monkey
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Alternative names
Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human Hsp27 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/300
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Validations
Western blot detection of Hsp27 in Hela,HsHela,A431,COS7,Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted).Predicted band size: 23KDa.Observed band size:27KDa.
Immunocytochemistry stain of Hela using Hsp27 mouse mAb (1:300).
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Background
Swiss-Prot Acc.P04792.Involved in stress resistance and actin organization.
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References
- Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"