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Product Name
Anti-Hsp27 (5D7) Mouse antibody
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Description
Hsp27 (5D7) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human HSP27 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of Hsp27 in Jurkat,HepG2 and Hela cell lysates using Hsp27 mouse mAb (dilution 1:500).Predicted band size:27KDa.Observed band size:27KDa.
Immunocytochemistry of HeLa cells using Hsp27 mouse mAb (dilution 1:100).
Immunoprecipitation analysis of Hela cell lysates using Hsp27 mouse mAb.
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Background
Swiss-Prot Acc.P04792.The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"