Anti-Phospho-AMPK alpha 1 (Ser496) Rabbit antibody

Cat.#: 167143

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Product Information

  • Product Name
    Anti-Phospho-AMPK alpha 1 (Ser496) Rabbit antibody
  • Documents
  • Description
    Phospho-AMPK alpha 1 (Ser496) Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Peptide sequence around phosphorylation site of serine 496 (S-G- pS-V-S) derived from Human AMPKα1.
  • Clonality
    Polyclonal
  • Formulation
    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB:1/500-1/1000,ICC:1/100-1/200,IHC:1/50-1/100

  • Validations

    Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKu03b11 (Phospho-Ser487)Antibody .

    Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKu03b11 (Phospho-Ser487)Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells using AMPKu03b11(Phospho-Ser487)Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells using AMPKu03b11(Phospho-Ser487)Antibody .

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKu03b11 (Phospho-Ser487)Antibody  (left) or the same antibody preincubated with blocking peptide (right).

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKu03b11 (Phospho-Ser487)Antibody (left) or the same antibody preincubated with blocking peptide (right).

    Western blot detection of AMPKa1(Phospho-Ser496) in Hela cells untreated or UV-treated, using AMPKa1(Phospho-Ser496) Rabbit pAb (dilution 1:2000, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:63kDa.Observed band size:63kDa.

    Western blot detection of AMPKa1(Phospho-Ser496) in Hela cells untreated or UV-treated, using AMPKa1(Phospho-Ser496) Rabbit pAb (dilution 1:2000, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:63kDa.Observed band size:63kDa.

  • Background
    Swiss-Prot Acc.Q13131.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"