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Product Name
Anti-Phospho-AMPK alpha 1 (Ser496) Rabbit antibody
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Description
Phospho-AMPK alpha 1 (Ser496) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 496 (S-G- pS-V-S) derived from Human AMPKα1.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000，ICC:1/100-1/200，IHC:1/50-1/100
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Validations
Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKu03b11 (Phospho-Ser487)Antibody .
Immunofluorescence staining of methanol-fixed Hela cells using AMPKu03b11(Phospho-Ser487)Antibody .
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKu03b11 (Phospho-Ser487)Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot detection of AMPKa1(Phospho-Ser496) in Hela cells untreated or UV-treated, using AMPKa1(Phospho-Ser496) Rabbit pAb (dilution 1:2000, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:63kDa.Observed band size:63kDa.
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Background
Swiss-Prot Acc.Q13131.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"