AMPK alpha 1 antibody

Cat.#: 108006

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Product Information

  • Product Name
    AMPK alpha 1 antibody
  • Documents
  • Description
    AMPK alpha 1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue. Positive WB detected in HeLa cells, rat heart tissue. Observed molecular weight by Western-blot: 63 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AMPK antibody; AMPK alpha antibody; AMPK alpha 1 antibody; AMPK subunit alpha 1 antibody; AMPK1 antibody; AMPKa1 antibody; PRKAA1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of AMPK alpha 1 recombinant protein (Accession Number: BC012622). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108006(PRKAA1 antibody) at dilution of 1:300

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108006(PRKAA1 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:108006(PRKAA1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:108006(PRKAA1 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:108006(AMPK alpha Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:108006(AMPK alpha Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms produced by alternative splicing.
  • References
    • Zhu K, Chen X, Liu J, Ye H, Zhu L, Wu JY. AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth. Protein & cell. 4(2):155-61. 2013.
    • Ye X, Zhang Y, He B, Meng Y, Li Y, Gao Y. Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration. International journal of clinical and experimental pathology. 8(12):15836-44. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"