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Product Name
AMPK alpha 1 antibody
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Description
AMPK alpha 1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue. Positive WB detected in HeLa cells, rat heart tissue. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMPK antibody; AMPK alpha antibody; AMPK alpha 1 antibody; AMPK subunit alpha 1 antibody; AMPK1 antibody; AMPKa1 antibody; PRKAA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AMPK alpha 1 recombinant protein (Accession Number: BC012622). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108006(PRKAA1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:108006(PRKAA1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:108006(AMPK alpha Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms produced by alternative splicing.
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References
- Zhu K, Chen X, Liu J, Ye H, Zhu L, Wu JY. AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth. Protein & cell. 4(2):155-61. 2013.
- Ye X, Zhang Y, He B, Meng Y, Li Y, Gao Y. Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration. International journal of clinical and experimental pathology. 8(12):15836-44. 2015.
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