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Product Name
Anti-AMPK alpha 1 Rabbit antibody
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Description
AMPK alpha 1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human AMPK alpha 1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
FC: 1/50
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Validations
Immunohistochemical analysis of paraffin-embedded human kidney, using AMPK alpha 1 Antibody.
Immunofluorescent analysis of Hela cells, using AMPK alpha 1 Antibody .
Western blot analysis of AMPK alpha 1 expression in (1) HeLa cell lysate; (2) HepG2 cell lysate.
Western blot analysis of AMPK alpha 1 expression in C6,K562,HepG2,3T3 and Hela cell lysates using AMPK alpha 1 antibody at 1/1000 dilution.Predicted band size:63KDa.Observed band size:63KDa.
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Background
Swiss-Prot Acc.Q13131.AMPKA1 a protein kinase of the CAMKL family that plays a central role in regulating cellular and organismal energy balance in response to the balance between AMP/ATP, and intracellular Ca(2+) levels.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"