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Anti-NFkB p105/p50 (5D5) Mouse antibody

Cat.#: 168315

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-NFkB p105/p50 (5D5) Mouse antibody
  • Documents
  • Description
    NFkB p105/p50 (5D5) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human
  • Isotype
    Mouse IgG2a
  • Preparation
    Antigen: Purified recombinant fragment of human NFKB1 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining.

    Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and negative control (purple).

    Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and negative control (purple).

    Western blot detection of NF-κB p105/p50 in THP-1,MCF7,HCT116,Jurkat and Raji cell lysates using NF-κB p105/p50 mouse mAb (1:5000 diluted).Predicted band size:50/105KDa.Observed band size:50/105KDa.

    Western blot detection of NF-κB p105/p50 in THP-1,MCF7,HCT116,Jurkat and Raji cell lysates using NF-κB p105/p50 mouse mAb (1:5000 diluted).Predicted band size:50/105KDa.Observed band size:50/105KDa.

  • Background
    Swiss-Prot Acc.P19838.This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"