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Product Name
Anti-NADPH Oxidase 4 Rabbit antibody
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Description
NADPH Oxidase 4 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human NOX4
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Western blot analysis of NOX4 expression in JAR cell lysate.
Immunohistochemical analysis of paraffin-embedded rat kidney, using NOX4 Antibody .
Immunofluorescent analysis of HeLa cells, using NOX4 Antibody .
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Background
Swiss-Prot Acc.Q9NPH5.The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"