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NOX4 antibody

Cat.#: 113314

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Product Information

  • Product Name
    NOX4 antibody
  • Documents
  • Description
    NOX4 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive FC detected in HeLa cells. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, BxPC-3 cells, HEK-293 cells. Observed molecular weight by Western-blot: 62kd
  • Tested applications
    ELISA, WB, IHC, IP, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    Kidney oxidase 1 antibody; KOX antibody; KOX 1 antibody; NADPH oxidase 4 antibody; NOX4 antibody; Renal NAD(P)H oxidase antibody; RENOX antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NOX4 recombinant protein (Accession Number: NM_016931). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113314(NOX4 antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113314(NOX4 antibody) at dilution of 1:600

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113314(NOX4 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113314(NOX4 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113314(NOX4 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113314(NOX4 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-NOX4 (IP:Catalog No:113314, 3ug; Detection:Catalog No:113314 1:1000) with HEK-293 cells lysate 1700ug.

    IP Result of anti-NOX4 (IP:Catalog No:113314, 3ug; Detection:Catalog No:113314 1:1000) with HEK-293 cells lysate 1700ug.

    1X10^6 HeLa cells were stained with 0.2ug NOX4 antibody (Catalog No:113314, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug NOX4 antibody (Catalog No:113314, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    NOX4 (NADPH oxidase 4 ) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.NOX4 has 7 isforms and the molecular weight are 7KDa,26-32KDa,58-67KDa,75-80KDa(PMID:11728818).
  • References
    • Ginnan R, Jourd'heuil FL, Guikema B, Simons M, Singer HA, Jourd'heuil D. NADPH oxidase 4 is required for interleukin-1β-mediated activation of protein kinase Cδ and downstream activation of c-jun N-terminal kinase signaling in smooth muscle. Free radical biology & medicine. 54:125-34. 2013.
    • Jin Y, Liu K, Peng J. Rhizoma Dioscoreae Nipponicae polysaccharides protect HUVECs from H2O2-induced injury by regulating PPARγ factor and the NADPH oxidase/ROS-NF-κB signal pathway. Toxicology letters. 232(1):149-158. 2014.
    • Fu C, Xu D, Wang CY. Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats. Journal of cellular physiology. 230(9):2184-201. 2015.
    • Xue H, Zhou S, Xiao L, Guo Q, Liu S, Wu Y. Hydrogen sulfide improves the endothelial dysfunction in renovascular hypertensive rats. Physiological research / Academia Scientiarum Bohemoslovaca. 64(5):663-72. 2015.
    • Li Y, Wang W, Xu X, Sun S, Xu X, Qu XJ. {2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3. BioMed research international. 2015:491205. 2015.
    • Wang J, Shao M, Liu M. PKCα promotes generation of reactive oxygen species via DUOX2 in hepatocellular carcinoma. Biochemical and biophysical research communications. 463(4):839-45. 2015.
    • Xu G, Wu H, Zhang J. Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice. Free radical biology & medicine. 87:15-25. 2015.
    • Kim HJ, Magesh V, Lee JJ, Kim S, Knaus UG, Lee KJ. Ubiquitin C-terminal hydrolase-L1 increases cancer cell invasion by modulating hydrogen peroxide generated via NADPH oxidase 4. Oncotarget. 6(18):16287-303. 2015.

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