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Product Name
Anti-Nox4 antibody
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Description
Rabbit polyclonal antibody to Nox4
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse, Rat, Dog, Cow, Horse
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Alternative names
AI648021 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human nox-4 81-180/578
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
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Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Fig3: Sample: Hela Cell Lysate at 40 ug; Primary: Anti- NADPH oxidase 4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 64 kD; Observed band size: 64 kD
Fig4: Sample: HepG2 Cell Lysate at 40 ug; Primary: Anti- NADPH oxidase 4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 64 kD; Observed band size: 64 kD
Fig5: Sample: Lung (Mouse) Lysate at 40 ug; Primary: Anti- NADPH oxidase4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 64 kD; Observed band size: 64 kD
Fig6: Sample:; Cerebellum (Mouse) Lysate at 40 ug; Primary: Anti-NADPH oxidase 4 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 64 kD; Observed band size: 64 kD
Fig7: Blank control: Raji.; Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilutio
Fig8: Sample:; muscle (Mouse) Lysate at 40 ug; Primary: Anti-NADPH oxidase 4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 64 kD; Observed band size: 64 kD
Fig9: Sample:; heart (Mouse) Lysate at 40 ug; Primary: Anti-NADPH oxidase 4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 64 kD; Observed band size: 64 kD
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