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Product Name
Anti-LIN28A (9G2) Mouse antibody
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Description
LIN28A (9G2) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western blot detection of LIN28A in NTERA2 cell lysates using LIN28A (9G2) Mouse mAb(1:1000 diluted).Predicted band size:23KDa.Observed band size:23KDa.
Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Western blot analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).
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Background
Swiss-Prot Acc.Q9H9Z2.LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.
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