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LIN28 antibody

Cat.#: 112231

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Product Information

  • Product Name
    LIN28 antibody
  • Documents
  • Description
    LIN28 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in human embronic stem cells. Positive WB detected in K-562 cells, NCCIT cell. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 28 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CSDD1 antibody; FLJ12457 antibody; LIN 28 antibody; lin 28 homolog (C. elegans) antibody; Lin 28A antibody; LIN28 antibody; Lin28A antibody; Protein lin 28 homolog A antibody; ZCCHC1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of LIN28 recombinant protein (Accession Number: NM_024674). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112231(LIN28 antibody) at dilution of 1:300

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112231(LIN28 antibody) at dilution of 1:300

    IP Result of anti-LIN28 (IP:Catalog No:112231, 3ug; Detection:Catalog No:112231 1:500) with K-562 cells lysate 1000ug.

    IP Result of anti-LIN28 (IP:Catalog No:112231, 3ug; Detection:Catalog No:112231 1:500) with K-562 cells lysate 1000ug.

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112231(LIN28 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112231(LIN28 antibody) at dilution of 1:50 (under 40x lens)

    Confocal immunofluorescent analysis of human embronic stem cells with Catalog No:112231 at dilution of 1:200. The PE shows staining with Catalog No:112231/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI.(10X)

    Confocal immunofluorescent analysis of human embronic stem cells with Catalog No:112231 at dilution of 1:200. The PE shows staining with Catalog No:112231/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI.(10X)

    Confocal immunofluorescent analysis of human embronic stem cells with Catalog No:112231 at dilution of 1:400. The PE shows staining with Catalog No:112231/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X)

    Confocal immunofluorescent analysis of human embronic stem cells with Catalog No:112231 at dilution of 1:400. The PE shows staining with Catalog No:112231/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X)

  • Background
    LIN28 is one of the four key human factors (ocT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin.
  • References
    • You X, Liu F, Zhang T. Hepatitis B virus X protein upregulates Lin28A/Lin28B through Sp-1/c-Myc to enhance the proliferation of hepatoma cells. Oncogene. 33(4):449-60. 2014.
    • Hayashi S, Tanaka J, Okada S. Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Experimental cell research. 319(8):1220-8. 2013.
    • Piazzon N, Schlotter F, Lefebvre S. Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle. Nucleic acids research. 41(2):1255-72. 2013.
    • Cimadamore F, Amador-Arjona A, Chen C, Huang CT, Terskikh AV. SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors. Proceedings of the National Academy of Sciences of the United States of America. 110(32):E3017-26. 2013.
    • Teng RY, Zhou JC, Jiang ZN. The relationship between Lin28 and the chemotherapy response of gastric cancer. OncoTargets and therapy. 6:1341-5. 2013.
    • Yin F, Lan R, Zhang X. LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16. Molecular and cellular biology. 34(2):158-79. 2014.
    • Wu T, Jia J, Xiong X. Increased expression of Lin28B associates with poor prognosis in patients with oral squamous cell carcinoma. PloS one. 8(12):e83869. 2013.
    • Tan SM, Altschuler G, Zhao TY. Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation. Nucleic acids research. 42(12):7997-8007. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"