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Product Name
Anti-Ku70 (1D3) Mouse antibody
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Description
Ku70 (1D3) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human XRCC6 (AA: 6-214) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of Ku70 in Jurkat,A549 and MCF7 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Immunofluorescence analysis of MCF-7 cells using Ku70 mouse mAb (green). Blue
Flow cytometric analysis of A431 cells using Ku70 mouse mAb (green) and negative control (red).
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Background
Swiss-Prot Acc.P12956.The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"