Anti-Ku70 (7B1) Mouse antibody

Cat.#: 168197

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Product Information

  • Product Name
    Anti-Ku70 (7B1) Mouse antibody
  • Documents
  • Description
    Ku70 (7B1) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP, ChIP
  • Species reactivity
    Human, Monkey
  • Alternative names
    5''-deoxyribose-5-phosphate lyase Ku70;5''-dRP lyase Ku70;70 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1;ATP dependent DNA helicase II 70 kDa subunit;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human Ku70 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/200

  • Validations

    Immunoprecipitation analysis of Hela cell lysates using Ku70 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using Ku70 mouse mAb.

    Western blot detection of Ku70 in Hela,A549,COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.

    Western blot detection of Ku70 in Hela,A549,COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using anti-Ku70 antibody (dilution 1:200).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using anti-Ku70 antibody (dilution 1:200).

  • Background
    Swiss-Prot Acc.P12956.It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"