Anti-Ku70 (6H10) Mouse antibody

Cat.#: 168449

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Product Information

  • Product Name
    Anti-Ku70 (6H10) Mouse antibody
  • Documents
  • Description
    Ku70 (6H10) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human
  • Alternative names
    5''-deoxyribose-5-phosphate lyase Ku70;5''-dRP lyase Ku70;70 kDa subunit of Ku antigen;ATP dependent DNA helicase 2 subunit 1;ATP dependent DNA helicase II 70 kDa subunit;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit; antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant human Ku70 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/200

  • Validations

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using Ku70 mouse mAb (dilution 1:200).

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using Ku70 mouse mAb (dilution 1:200).

    Western blot detection of Ku70 in Hela,A549,MCF7,Jurkat and Raji cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

    Western blot detection of Ku70 in Hela,A549,MCF7,Jurkat and Raji cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

  • Background
    Swiss-Prot Acc.P12956.It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"