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Product Name
Anti-Hsp90 alpha (7F7) Mouse antibody
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Description
Hsp90 alpha (7F7) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human HSP90AA1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of HeLa cells using HSP90AA1 mouse mAb (green) and negative control (red).
Western blot analysis using HSP90AA1 mouse mAb against NIH3T3 (1), HeLa (2), HCT116(3), HL-60 (4) and C0S7 (5) cell lysate.
Western blot detection of HSP90AA1 in CHO-K1,Jurkat,MCF7,COS7,C6,3T3 and Hela cell lysates using HSP90AA1 mouse mAb (1:1000 diluted).Predicted band size:90KDa.Observed band size:90KDa.
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP90AA1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using HSP90AA1 mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.P07900.HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"