Anti-HPRT (1E9) Mouse antibody
HPRT (1E9) Mouse monoclonal antibody
WB, IHC-P, FC
Antigen: Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli.
Purified antibody in PBS with 0.05% sodium azide.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
Western blot detection of HPRT1 in A549,3T3 and Hela cell lysates using HPRT1 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.
Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
Immunohistochemical analysis of paraffin-embedded kidney tissues using HPRT1 mouse mAb with DAB staining.
Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.
Swiss-Prot Acc.P00492.The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
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