Anti-HPRT (1E9) Mouse antibody

Cat.#: 168483

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Product Information

  • Product Name
    Anti-HPRT (1E9) Mouse antibody
  • Documents
  • Description
    HPRT (1E9) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified antibody in PBS with 0.05% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot detection of HPRT1 in A549,3T3 and Hela cell lysates using HPRT1 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.

    Western blot detection of HPRT1 in A549,3T3 and Hela cell lysates using HPRT1 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.

    Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).

    Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).

    Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.

    Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.

    Immunohistochemical analysis of paraffin-embedded kidney tissues using HPRT1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded kidney tissues using HPRT1 mouse mAb with DAB staining.

    Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.

    Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.

  • Background
    Swiss-Prot Acc.P00492.The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"