HPRT1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human brain tissue, human pancreas tissue. Positive FC detected in HeLa cells. Positive WB detected in HepG2 cells, A549 cells, HeLa cells, Jurkat cells, MCF7 cells, mouse liver tissue, NIH/3T3 cells, rat brain tissue, Y79 cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 24-28 kDa
ELISA, IHC, IF, FC, IP, WB
Human,Mouse,Rat; other species not tested.
HGPRT antibody; HGPRTase antibody; HPRT antibody; HPRT1 antibody
This antibody was obtained by immunization of HPRT1 recombinant protein (Accession Number: NM_000194). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111450(HPRT1 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human brain using Catalog No:111450(HPRT1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:111450(HPRT1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using HPRT1 antibody Catalog No:111450 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HPRT1 (IP:Catalog No:111450, 4ug; Detection:Catalog No:111450 1:1000) with mouse liver tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug HPRT1 antibody (Catalog No:111450, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
HPRT1 represents hypoxanthine phosphoribosyltransferase 1
- Owens GC, Huynh MN, Chang JW. Differential expression of interferon-γ and chemokine genes distinguishes Rasmussen encephalitis from cortical dysplasia and provides evidence for an early Th1 immune response. Journal of neuroinflammation. 10:56. 2013.
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