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Product Name
Anti-HPRT Rabbit antibody
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Description
HPRT Rabbit polyclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
HPRT; HGPRT antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human HPRT
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
IP: 1/20
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Validations
Western blot detection of HPRT in Hela,CHO-K1,C6,Rat Brain,Jurkat cell lysates using HPRT Rabbit pAb(1:500 diluted).Predicted band size:25kDa.Observed band size:25kDa.
Western blot detection of HPRT in K562,3T3,Hela cell lysates using HPRT Rabbit pAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.
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Background
Swiss-Prot Acc.P00492.Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"