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Product Name
Anti-HP1 alpha (3G2) Mouse antibody
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Description
HP1 alpha (3G2) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IHC-P
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Species reactivity
Human, Mouse
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Alternative names
Antigen p25;Cbx 5;Cbx5;CBX5_HUMAN;CG8409;Chromobox homolog 5 (Drosophila HP1 alpha); Chromobox homolog 5 (HP1 alpha homolog Drosophila); Chromobox homolog 5 (HP1 alpha homolog antibody; Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Chromobox prote antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human HP1 alpha protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/300
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Validations
Immunocytochemistry stain of Hela using HP1 alpha mouse mAb (1:300).
Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa
Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).
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Background
Swiss-Prot Acc.P45973.Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
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