-
Product Name
Anti-HP1 alpha Rabbit antibody
- Documents
-
Description
HP1 alpha Rabbit monoclonal antibody
-
Tested applications
WB, IHC-P, ICC/IF, FC, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
HP1; HP1A; HEL25 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human HP1 alpha
-
Clonality
Monoclonal
-
Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
-
Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
1:2000-1:10000
1:50
1:50
1:20
1:20
-
Validations
Immunocytochemistry of HP1 alpha(green) in Hela cells using HP1 alpha Rabbit mAb at dilution 1/50, and DAPI(blue)
Western blot detection of HP1 alpha in 3T3,Hela cell lysates using HP1 alpha Rabbit mAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:22kDa.
-
Background
Swiss-Prot Acc.P45973.Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"