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Product Name
Anti-HP1 alpha (1E8) Mouse antibody
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Description
HP1 alpha (1E8) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Rat, Mouse
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant protein expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1;000
IHC: 1/100-200
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Validations
Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α Mouse mAb diluted at 1:200.
Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1α Mouse mAb diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 alpha; Mouse mAb diluted at 1:200
Western blot detection of HP1α in COS7,Hela cell lysates using HP1α (1E8) Mouse mAb(1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.
Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α Mouse mAb diluted at 1:200.
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Background
Swiss-Prot Acc.P45973.Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"