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Product Name
Anti-ENO1 (3F7) Mouse antibody
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Description
ENO1 (3F7) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat, Monkey
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Alternative names
2 phospho D glycerate hydro lyase; 2-phospho-D-glycerate hydro-lyase; Alpha enolase; Alpha enolase like 1; Alpha-enolase; C myc promoter binding protein; C-myc promoter-binding protein; EC 4.2.1.11; eno1; ENO1L1; ENOA_HUMAN; Enolase 1 (alpha); Enolase 1 ( antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human Enolase-1 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000
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Validations
Western blot detection of Enolase-1 in T47D,3T3,COS7,C6 and Hela cell lysates using Enolase-1 mouse mAb(dilution 1:2000).Predicted band size:47kDa.Observed band size:47kDa.
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Background
Swiss-Prot Acc.P06733.Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"