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ENO1 antibody

Cat.#: 110253

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Product Information

  • Product Name
    ENO1 antibody
  • Documents
  • Description
    ENO1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human liver cancer tissue, human stomach cancer tissue. Positive FC detected in HeLa cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in human skeletal muscle tissue, PC-3 cells, SGC-7901 cells. Observed molecular weight by Western-blot: 47 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Alpha enolase antibody; C myc promoter binding protein antibody; ENO1 antibody; ENO1L1 antibody; Enolase 1 antibody; enolase 1 antibody; (alpha) antibody; MBP 1 antibody; MBPB1 antibody; MPB 1 antibody; MPB1 antibody; NNE antibody; Non neural enolase antibody; Phosphopyruvate hydratase antibody; Plasminogen binding protein antibody; PPH antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ENO1 recombinant protein (Accession Number: NM_001428). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:110253(ENO1 antibody) at dilution of 1:500

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:110253(ENO1 antibody) at dilution of 1:500

    IP Result of anti-ENO1 (IP:Catalog No:110253, 3ug; Detection:Catalog No:110253 1:500) with mouse skeletal muscle tissue lysate 3500ug.

    IP Result of anti-ENO1 (IP:Catalog No:110253, 3ug; Detection:Catalog No:110253 1:500) with mouse skeletal muscle tissue lysate 3500ug.

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110253(ENO1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110253(ENO1 antibody) at dilution of 1:50 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using ENO1 antibody Catalog No:110253 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using ENO1 antibody Catalog No:110253 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    1X10^6 HeLa cells were stained with 0.2ug ENO1 antibody (Catalog No:110253, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HeLa cells were stained with 0.2ug ENO1 antibody (Catalog No:110253, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)
  • References
    • Lu Y, Guo J, Di Y, Zong Y, Qu S, Tian J. Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells. Molecules and cells. 28(3):175-81. 2009.
    • Liu CI, Wang RY, Lin JJ. Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway. Journal of proteomics. 75(18):5578-89. 2012.
    • Liu C, Zhang A, Guo J. Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics. Journal of proteome research. 11(11):5396-405. 2012.
    • Liu Z, Chen C, Yang H. Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma. Proteomics. 12(22):3416-25. 2012.
    • Choi M, Choi JW, Chaudhari HN, Aseer KR, Mukherjee R, Yun JW. Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 31(2-3):408-20. 2013.
    • Gao S, Li H, Cai Y. Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis. Archives of biochemistry and biophysics. 542:46-55. 2014.
    • Song Y, Luo Q, Long H. Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma. Molecular cancer. 13:65. 2014.
    • Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"