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Product Name
Anti-ENO1 Rabbit antibody
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Description
ENO1 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human ENO1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Immunohistochemical analysis of paraffin-embedded human colon, using ENO1 Antibody.
Western blot analysis of ENO1 expression in SKBR3,Jurkat,MCF7,3T3 and Hela cell lysates using ENO1 antibody at 1/3000 dilution.Predicted band size:47KDa.Observed band size:47KDa.
Western blot analysis of ENO1 in (1)MCF-7 whole cell lysate; (2)Rat brain tissue lysate.
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Background
Swiss-Prot Acc.P06733.Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"