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Product Name
Anti-DiMethyl-Histone H3 (Lys27) (5F6) Mouse antibody
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Description
DiMethyl-Histone H3 (Lys27) (5F6) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Synthetic Peptide of Histone H3 (Di Methyl Lys27)
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-3000
IP:1/200
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Validations
Western blot detection of Di-Methyl-Histone H3 in C6,3T3,C2C12 cell lysates using Di-Methyl-Histone H3 (Lys27) (5F6) Mouse mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
Western blot analysis of Hela, diluted at 1) 1:1000 . 2) 1:3000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunocytochemistry staining of HeLa cells using Di-Methyl-Histone H3 (Lys27) (5F6) Mouse mAb(dilution 1:100).
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
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Background
Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
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