Anti-DiMethyl-Histone H3 (Lys27) (5F6) Mouse antibody

Cat.#: 168162

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Product Information

  • Product Name
    Anti-DiMethyl-Histone H3 (Lys27) (5F6) Mouse antibody
  • Documents
  • Description
    DiMethyl-Histone H3 (Lys27) (5F6) Mouse monoclonal antibody
  • Tested applications
    WB, IP, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Synthetic Peptide of Histone H3 (Di Methyl Lys27)
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000-3000

    IP:1/200

  • Validations

    Western blot detection of Di-Methyl-Histone H3 in C6,3T3,C2C12 cell lysates using Di-Methyl-Histone H3 (Lys27) (5F6) Mouse mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

    Western blot detection of Di-Methyl-Histone H3 in C6,3T3,C2C12 cell lysates using Di-Methyl-Histone H3 (Lys27) (5F6) Mouse mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

    Western blot analysis of Hela, diluted at 1) 1:1000 . 2) 1:3000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

    Western blot analysis of Hela, diluted at 1) 1:1000 . 2) 1:3000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

    Immunocytochemistry staining of HeLa cells using Di-Methyl-Histone H3 (Lys27) (5F6) Mouse mAb(dilution 1:100).

    Immunocytochemistry staining of HeLa cells using Di-Methyl-Histone H3 (Lys27) (5F6) Mouse mAb(dilution 1:100).

    1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

    1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

  • Background
    Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"