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Product Name
Anti-MonoMethyl-Histone H3 (Lys9) (10F5) Mouse antibody
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Description
MonoMethyl-Histone H3 (Lys9) (10F5) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Synthetic Peptide of Histone H3 (Mono Methyl Lys9)
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-3000
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Validations
Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7, diluted at 1:1000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot detection of Mono-Methyl-Histone H3 in MCF7,C2C12,C6,3T3 cell lysates using Mono-Methyl-Histone H3 (Lys9) (10F5) Mouse mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
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Background
Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"