-
Product Name
Anti-DiMethyl-Histone H3 (Lys9) (10B2) Mouse antibody
- Documents
-
Description
DiMethyl-Histone H3 (Lys9) (10B2) Mouse monoclonal antibody
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Isotype
Mouse IgG1
-
Preparation
Antigen: Synthetic Peptide of Histone H3 (Di Methyl Lys9)
-
Clonality
Monoclonal
-
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/1000-3000
-
Validations
Western blot detection of Di-Methyl-Histone H3 in MCF7,C2C12,C6,3T3,Hela cell lysates using Di-Methyl-Histone H3 (Lys9) (10B2) Mouse mAb(1:1000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Raw264.7, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
-
Background
Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"