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Product Name
Anti-CREB (2F6) Mouse antibody
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Description
CREB (2F6) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human CREB1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.
Western blot detection of CREB in Jurkat,A549,MCF7,C6 and Hela cell lysates using CREB mouse mAb (1:3000 diluted).Predicted band size:43KDa.Observed band size:43KDa.
Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green). Red
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Background
Swiss-Prot Acc.P16220.This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"