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  • Anti-Caspase-8 (4C2) Mouse antibody
  • Anti-Caspase-8 (4C2) Mouse antibody
  • Anti-Caspase-8 (4C2) Mouse antibody

Anti-Caspase-8 (4C2) Mouse antibody

Cat.#: 168136

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-Caspase-8 (4C2) Mouse antibody
  • Documents
  • Description
    Caspase-8 (4C2) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IHC-P
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    IgG1
  • Preparation
    Antigen: Recombinant Protein of Caspase-8
  • Clonality
    Monoclonal antibody
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000-2000

    IHC:1/200-500

    IF: 1/200

  • Validations

    Western blot detection of Caspase-8 in K562,3T3,MCF7 and Jurkat cell lysates using Caspase-8 mouse mAb (1:2000 diluted).Predicted band size:57kDa.Observed band size:57kDa.

    Western blot detection of Caspase-8 in K562,3T3,MCF7 and Jurkat cell lysates using Caspase-8 mouse mAb (1:2000 diluted).Predicted band size:57kDa.Observed band size:57kDa.

    Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase8 uff08uff09Mouse mAb diluted at 1:500.

    Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase8 uff08uff09Mouse mAb diluted at 1:500.

    Western blot detection of Caspase-8 in THP-1,Rat Brain,Hela cell lysates using Caspase-8 (4C2) Mouse mAb(1:1000 diluted).Predicted band size:43,57KDa.Observed band size:54/43/18KDa.

    Western blot detection of Caspase-8 in THP-1,Rat Brain,Hela cell lysates using Caspase-8 (4C2) Mouse mAb(1:1000 diluted).Predicted band size:43,57KDa.Observed band size:54/43/18KDa.

  • Background
    Swiss-Prot Acc.Q14790.Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"