Anti-Caspase-8 (4C2) Mouse antibody
Caspase-8 (4C2) Mouse monoclonal antibody
WB, ICC/IF, IHC-P
Human, Mouse, Rat
Antigen: Recombinant Protein of Caspase-8
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Western blot detection of Caspase-8 in K562,3T3,MCF7 and Jurkat cell lysates using Caspase-8 mouse mAb (1:2000 diluted).Predicted band size:57kDa.Observed band size:57kDa.
Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase8 uff08uff09Mouse mAb diluted at 1:500.
Western blot detection of Caspase-8 in THP-1,Rat Brain,Hela cell lysates using Caspase-8 (4C2) Mouse mAb(1:1000 diluted).Predicted band size:43,57KDa.Observed band size:54/43/18KDa.
Swiss-Prot Acc.Q14790.Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
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