Product Name

Annexin A2 antibody

Documents

Description

Annexin A2 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human colon cancer tissue, human oesophagus cancer tissue. Positive IF detected in HeLa cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, K-562 cells, mouse placenta tissue, NIH/3T3 cells, rat uterus tissue. Observed molecular weight by Western-blot: 39 kDa

Tested applications

ELISA, IP, WB, IF, IHC

Species reactivity

Human, Mouse, Rat; other species not tested.

Alternative names

Annexin 2 antibody; annexin A2 antibody; Annexin II antibody; ANX2 antibody; ANX2L4 antibody; ANXA2 antibody; CAL1H antibody; Calpactin 1 heavy chain antibody; Calpactin I heavy chain antibody; Chromobindin 8 antibody; LIP2 antibody; LipOCortin II antibody; LPC2 antibody; LPC2D antibody; P36 antibody; PAP IV antibody; Protein I antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Annexin A2 recombinant protein (Accession Number: XM_017022090). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:200-1:2000

IHC: 1:50-1:500

IF: 1:50-1:500

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108093(Annexin A2 Antibody) at dilution of 1:2000

Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:108093(Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:108093(Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:108093(Annexin A2 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IP Result of anti-ANXA2 (IP:Catalog No:108093, 3ug; Detection:Catalog No:108093 1:500) with HeLa cells lysate 3000ug.

Background

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

References

Zhuang H, Tan M, Liu J. Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. Molecular cancer. 13:243. 2014.
Li J, Guo D, Huang L. The interaction between host Annexin A2 and viral Nsp9 is beneficial for replication of porcine reproductive and respiratory syndrome virus. Virus research. 189:106-13. 2014.
Lu CM, Lin JJ, Huang HH. A panel of tumor markers, calreticulin, annexin A2, and annexin A3 in upper tract urothelial carcinoma identified by proteomic and immunological analysis. BMC cancer. 14:363. 2014.
Zhuang H, Tan M, Liu J. The expression of annexin II and Lewis y antigen in ovarian epithelial tumors and the correlation between them. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(4):2343-9. 2015.
Chen P, Yan H, Tian Y. Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease. Scientific reports. 5:8162. 2015.
Dai J, Zhan C, Xu W. Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis. Andrology. 3(5):967-78. 2015.
Renner B, Tong HH, Laskowski J. Annexin A2 Enhances Complement Activation by Inhibiting Factor H. Journal of immunology (Baltimore, Md. : 1950). 196(3):1355-65. 2016.
Minamide LS, Maiti S, Boyle JA. Isolation and characterization of cytoplasmic cofilin-actin rods. The Journal of biological chemistry. 285(8):5450-60. 2010.

Annexin A2 antibody

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