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Product Name
Annexin A2 antibody
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Description
Annexin A2 Mouse Monoclonal antibody. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells. Positive IP detected in HeLa cells. Positive FC detected in HeLa cells. Positive IHC detected in human breast cancer tissue, human gliomas tissue. Positive IF detected in MCF-7 cells, Hela cells. Observed molecular weight by Western-blot: 35-38 kDa
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Tested applications
ELISA, WB, IHC, IP, FC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Annexin 2 antibody; annexin A2 antibody; Annexin II antibody; ANX2 antibody; ANX2L4 antibody; ANXA2 antibody; CAL1H antibody; Calpactin 1 heavy chain antibody; Calpactin I heavy chain antibody; Chromobindin 8 antibody; LIP2 antibody; LipOCortin II antibody; LPC2 antibody; LPC2D antibody; P36 antibody; PAP IV antibody; Protein I antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Annexin A2 recombinant protein (Accession Number: XM_017022090). Purification method: Caprylic Acid/Ammonium Sulfate Precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107055(ANXA2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107055(ANXA2 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107055(ANXA2 Antibody) at dilution of 1:50
Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:107055(Annexin A2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
IP Result of anti-Annexin A2 (IP:Catalog No:107055, 4ug; Detection:Catalog No:107055 1:1500) with HeLa cells lysate 2400ug.
1X10^6 HeLa cells were stained with 0.2ug Annexin A2 antibody (Catalog No:107055, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"