Human ALK-4 / ACVR1B (His & Fc Tag) recombinant protein

Human ALK-4 / ACVR1B (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.

Activin receptor type-1B

ALK-4; 6820432J04; ACVR1B; ACTR-IB; SKR2; ACVRLK4; ACTRIB; ALK4

P36896

ACVR1B; ACVRLK4; ALK4

Human Cells

A DNA sequence encoding the human ACVR1B (NP_004293.1) extracellular domain (Met 1-Glu 126) was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human ALK4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 351 amino acids and has a predicted molecular mass of 39.6 kDa. In SDS-PAGE under reducing conditions, rhALK4/Fc monomer migrates as an approximately 46 kDa band due to glycosylation, with ~15% free Fc fragment.

> 80 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 μg/ml (100 μl/well) can bind human ALK-4 with a linear range of 0.0068-0.16 μg/ml.