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  • YWHAH antibody
  • YWHAH antibody
  • YWHAH antibody

YWHAH antibody

Cat.#: 116898

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Product Information

  • Product Name
    YWHAH antibody
  • Documents
  • Description
    YWHAH Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, A431 cells, mouse brain tissue, NIH/3T3 cells, Raji cells. Positive IP detected in NIH/3T3 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 28 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    14 3 3 protein eta antibody; 14-3-3 antibody; 14-3-3 eta antibody; Protein AS1 antibody; YWHA1 antibody; YWHAH antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of YWHAH recombinant protein (Accession Number: BC003047). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116898(YWHAH antibody) at dilution of 1:300

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116898(YWHAH antibody) at dilution of 1:300

    Immunofluorescent analysis of Hela cells, using YWHAH antibody Catalog No:116898 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using YWHAH antibody Catalog No:116898 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-YWHAH (IP:Catalog No:116898, 4ug; Detection:Catalog No:116898 1:1000) with NIH/3T3 cells lysate 1600ug.

    IP Result of anti-YWHAH (IP:Catalog No:116898, 4ug; Detection:Catalog No:116898 1:1000) with NIH/3T3 cells lysate 1600ug.

  • Background
    YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"