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Product Name
YWHAH antibody
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Description
YWHAH Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, A431 cells, mouse brain tissue, NIH/3T3 cells, Raji cells. Positive IP detected in NIH/3T3 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
14 3 3 protein eta antibody; 14-3-3 antibody; 14-3-3 eta antibody; Protein AS1 antibody; YWHA1 antibody; YWHAH antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of YWHAH recombinant protein (Accession Number: NM_003405). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116898(YWHAH antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using YWHAH antibody Catalog No:116898 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-YWHAH (IP:Catalog No:116898, 4ug; Detection:Catalog No:116898 1:1000) with NIH/3T3 cells lysate 1600ug.
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Background
YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"