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Anti-YWHAH antibody

Cat.#: 104413

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Product Information

  • Product Name
    Anti-YWHAH antibody
  • Documents
  • Description
    Mouse monoclonal to YWHAH
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human 14-3-3 eta / YWHAH
    No cross-reactivity in ELISA with E.coli cell lysate
  • Alternative names
    YWHA1 antibody; 14-3-3 protein eta antibody; Ywhah antibody; OTTMUSP00000017560 antibody; 14-3-3 eta antibody; 14-3-3 eta antibody; LL22NC03-44A4.1 antibody; YWHA1 antibody; YWHAH antibody
  • Immunogen
    Human YWHAH (GST Tag) recombinant protein
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 0.5-2 μg/mL

    ELISA: 0.5-1 μg/mL

    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human YWHAH. The detection limit for Human YWHAH is approximately 0.078 ng/well.

    IP: 2-8 μg/mg of lysate

  • Validations

    14-3-3 eta / YWHAH Antibody, Mouse MAb, Immunoprecipitation

    14-3-3 eta / YWHAH Antibody, Mouse MAb, Immunoprecipitation

    14-3-3 eta / YWHAH Antibody, Mouse MAb, Western blot

    14-3-3 eta / YWHAH Antibody, Mouse MAb, Western blot

  • References
    • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
    • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
    • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
    • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"