XRCC5/Ku80 antibody

XRCC5/Ku80 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

XRCC5/Ku80 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HepG2 cells, A431 cells, HEK-293 cells, HeLa cells, human liver tissue, K-562 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 80 kDa

ELISA, WB, IHC, IF, IP

Human; other species not tested.

86 kDa subunit of Ku antigen antibody; CTC85 antibody; CTCBF antibody; DNA repair protein XRCC5 antibody; FLJ39089 antibody; G22P2 antibody; KARP 1 antibody; KARP1 antibody; Ku80 antibody; Ku86 antibody; KUB2 antibody; NFIV antibody; Nuclear factor IV antibody; Thyroid lupus autoantigen antibody; TLAA antibody; XRCC5 antibody

Rabbit IgG

This antibody was obtained by immunization of XRCC5/Ku80 recombinant protein (Accession Number: NM_021141). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.

• Dong H, Shi Q, Song X. Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. Toxicology and applied pharmacology. 286(1):10-6. 2015.