XRCC5/Ku80 antibody

Cat.#: 116876

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Product Information

  • Product Name
    XRCC5/Ku80 antibody
  • Documents
  • Description
    XRCC5/Ku80 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HepG2 cells, A431 cells, HEK-293 cells, HeLa cells, human liver tissue, K-562 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 80 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    86 kDa subunit of Ku antigen antibody; CTC85 antibody; CTCBF antibody; DNA repair protein XRCC5 antibody; FLJ39089 antibody; G22P2 antibody; KARP 1 antibody; KARP1 antibody; Ku80 antibody; Ku86 antibody; KUB2 antibody; NFIV antibody; Nuclear factor IV antibody; Thyroid lupus autoantigen antibody; TLAA antibody; XRCC5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of XRCC5/Ku80 recombinant protein (Accession Number: NM_021141). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116876(XRCC5 antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116876(XRCC5 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116876(XRCC5 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116876(XRCC5 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116876(XRCC5 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116876(XRCC5 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using XRCC5 antibody Catalog No:116876 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using XRCC5 antibody Catalog No:116876 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-XRCC5 (IP:Catalog No:116876, 4ug; Detection:Catalog No:116876 1:1000) with HEK-293 cells lysate 1200ug.

    IP Result of anti-XRCC5 (IP:Catalog No:116876, 4ug; Detection:Catalog No:116876 1:1000) with HEK-293 cells lysate 1200ug.

  • Background
    There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.
  • References
    • Dong H, Shi Q, Song X. Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. Toxicology and applied pharmacology. 286(1):10-6. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"