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  • Anti-Ku80 (8H1) Mouse antibody
  • Anti-Ku80 (8H1) Mouse antibody
  • Anti-Ku80 (8H1) Mouse antibody

Anti-Ku80 (8H1) Mouse antibody

Cat.#: 168094

Special Price 123.9 USD

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Product Information

  • Product Name
    Anti-Ku80 (8H1) Mouse antibody
  • Documents
  • Description
    Ku80 (8H1) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP, ChIP
  • Species reactivity
    Human, Monkey
  • Alternative names
    ATP dependent DNA helicase II 80 kDa subunit; ATP dependent DNA helicase II 86 Kd subunit; ATP dependent DNA helicase II; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box binding factor 85 kDa; CTC box-binding antibody
  • Isotype
    IgG1
  • Preparation
    Antigen: Purified recombinant human Ku80 protein fragments expressed in E.coli
  • Clonality
    Monoclonal antibody
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/400

    ;IP: 1/100

  • Validations

    Immunoprecipitation analysis of Hela cell lysates using Ku80 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using Ku80 mouse mAb.

    Western blot detection of Ku80 in COS7,Jurkat,Hela,MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.

    Western blot detection of Ku80 in COS7,Jurkat,Hela,MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.

    Immunofluorescent analysis of Hela cells using Ku80 mouse mAb (1:400).

    Immunofluorescent analysis of Hela cells using Ku80 mouse mAb (1:400).

  • Background
    Swiss-Prot Acc.P13010.Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"