Mouse VEGFR3/FLT-4 (Fc Tag) recombinant protein

Mouse VEGFR3/FLT-4 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Vascular endothelial growth factor receptor 3

RP23-58E13.1; VEGFR-3; VEGFR3; PCL; CHY; FLT41; AI323512; LMPH1A; FLT-4; FLT4

P35917

Flt4; Flt-4; Vegfr3

Human Cells

A DNA sequence encoding the mouse FLT4 (P35917-1) extracellular domain (Met 1-Glu 775) was was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse FLT4/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 992 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation and proteolytic cleavage, rm FLT4/Fc monomer migrates as three bands (150, 85, 65 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

1. Measured by its ability to bind human VEGF-D (Cat: 501854) in functional ELISA.
2. Immobilized human VEGF-C (Cat: 500116) at 10 μg/mL (100 μL/well) can bind mouse VEGFR3-Fc.
The EC₅₀ of mouse VEGFR3-Fc is 0.008 μg/mL.
3. Measured by its ability to bind with mouse FIGF-His (Cat:503314) in a functional ELISA.