Human VEGFR3/FLT-4 (Fc Tag) recombinant protein

Human VEGFR3/FLT-4 (Fc Tag) recombinant protein

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Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Vascular endothelial growth factor receptor 3

VEGFR-3; FLT-4

P35916

FLT4; VEGFR3

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ile 776) of human VEGFR3 (NP_002011.2) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Human

The recombinant human VEGFR3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 990 amino acids and predicts a molecular mass of 111 kDa. As a result of glycosylation and proteolytic cleavage, rhVEGFR3/Fc migrates as three bands (160, 85, 75 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
1. Immobilized recombinant human VEGFR3 at 5 μg/ml (100 μl/well) can bind recombinant human VEGF-D at a linear range of 62.5-2000 ng/ml.
2. Immobilized recombinant human VEGF-C at 10 μg/ml (100 μl/well) can bind recombinant human VEGFR3 at a linear range of 0.64-80 ng/ml.
3. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.