Human VEGFR3/FLT-4 (His Tag) recombinant protein

Human VEGFR3/FLT-4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.

Vascular endothelial growth factor receptor 3

FLT4; FLT-4; LMPH1A; AI323512; FLT41; PCL; CHY; RP23-58E13.1; VEGFR3; VEGFR-3

P35916

FLT4; VEGFR3

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ile 776) of human VEGFR3 (NP_002011.2) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant human VEGF R3 consists of 763 amino acids and predicts a molecular mass of 86 kDa. As a result of glycosylation, rhVEGFR3 migrates as an approximately 130 kDa in non-reduced SDS-PAGE.

> 97 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human VEGF-C (Cat: 500116) at 10 μg/mL (100 μl/well) can bind human VEGFR3-his. The EC₅₀ of human VEGFR3-his is 0.011 μg/mL.
3. Measured by its ability to bind human VEGF-D (Cat: 501854) in a functional ELISA.