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Product Name
VANGL2 antibody
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Description
VANGL2 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HT-1080 cells, MCF7 cells, mouse brain tissue, rat brain tissue. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human stomach tissue. Observed molecular weight by Western-blot: 55-60 kDa, 65-70 kDa
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Tested applications
ELISA, IHC, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1215 antibody; Loop tail protein 1 homolog antibody; LPP1 antibody; LTAP antibody; STB1 antibody; STBM antibody; STBM1 antibody; Strabismus 1 antibody; Van Gogh like protein 2 antibody; Vang like protein 2 antibody; VANGL2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VANGL2 recombinant protein (Accession Number: BC103920). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116712(VANGL2 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human stomach slide using Catalog No:116712(VANGL2 Antibody) at dilution of 1:50
IP Result of anti-VANGL2 (IP:Catalog No:116712, 4ug; Detection:Catalog No:116712 1:1000) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:116712(VANGL2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.
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